FastQCFastQC Report
Sun 13 May 2018
LZ_M31.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M31.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17364349
Sequences flagged as poor quality0
Sequence length33
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3098311.7842937849268061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGTTTGAATATTTGCTACTACCACCAAGATC176080.10140316806578813No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG173820.10010165080188149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA333100.026.6850341
CGTCTGA349150.026.00843816
ACGTCTG349700.025.98683715
CGGAAGA351650.025.8996434
ACACGTC357550.025.43509913
CACACGT360200.025.28544812
GCACACG361750.025.24829911
ATCGGAA351100.025.2258242
AGCACAC365400.025.062310
CACGTCT363050.025.03453614
TCGGAAG354050.024.9885183
AGAGCAC370550.024.7249078
GAACTCC367150.024.70746221
AACTCCA371500.024.41829722
GAGCACA375850.024.3942139
CAGTCAC361250.024.2927827
AAGAGCA382200.024.0984087
ACTCCAG376300.024.05301323
GTCTGAA380650.023.95545417
TCCAGTC379300.023.76667825
Produced by FastQC (version 0.11.2)