FastQCFastQC Report
Sun 13 May 2018
LZ_M32.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M32.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21889816
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3863761.7650947819753258Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA416550.026.6634121
CGGAAGA437700.025.9923694
CGTCTGA437900.025.92506816
ACGTCTG439900.025.84101515
ATCGGAA433200.025.5090942
GCACACG449500.025.34127811
ACACGTC455550.024.98341613
TCGGAAG445000.024.8412083
AGCACAC460700.024.79818310
CACACGT459000.024.78092812
AGAGCAC464500.024.5952578
GAACTCC462100.024.5701921
CACGTCT463000.024.55758514
GAGCACA475550.024.0862629
AACTCCA472200.024.0847322
GTCTGAA473400.024.05795317
AAGAGCA479600.023.9841387
CAGTCAC460150.023.97036627
CTGAACT475550.023.95185919
ACTCCAG477600.023.72762323
Produced by FastQC (version 0.11.2)