FastQCFastQC Report
Sun 13 May 2018
LZ_M33.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M33.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19486762
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3059911.5702506142375012Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA330900.026.6816391
ACGTCTG350600.026.06238215
CGTCTGA350650.026.04299716
CGGAAGA353200.025.7592964
ATCGGAA347100.025.415532
GCACACG361850.025.29069911
ACACGTC361750.025.27124413
CACACGT364150.025.09356512
CACGTCT368100.024.8194814
TCGGAAG356550.024.7299883
AGCACAC373250.024.6555710
GAACTCC373100.024.4795721
AGAGCAC379150.024.2752118
AACTCCA378300.024.17513322
CAGTCAC370800.024.00154927
ACTCCAG382400.023.84180523
GAGCACA388450.023.7012259
GTCTGAA387250.023.65481217
AAGAGCA391600.023.6344327
TCCAGTC386050.023.48351325
Produced by FastQC (version 0.11.2)