FastQCFastQC Report
Sun 13 May 2018
LZ_M34.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M34.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23205079
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4285871.8469534191200125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATA262720.11321659366037927No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT254760.10978631014356814No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG251710.10847194271564428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA453600.026.7409041
CGTCTGA475050.026.13952816
ACGTCTG477600.026.0197515
ATCGGAA467600.025.8873082
CGGAAGA481450.025.878914
ACACGTC486800.025.5507913
CACACGT489800.025.38051412
GCACACG492900.025.25982911
TCGGAAG482000.025.1075533
CACGTCT495600.025.10168814
AGCACAC498350.025.05114610
AGAGCAC504700.024.8055028
GAACTCC499550.024.79824821
AACTCCA510150.024.31209422
AAGAGCA518650.024.2502337
CAGTCAC496750.024.10105327
GAGCACA520100.024.0736129
GTCTGAA518900.023.99822617
ACTCCAG517050.023.94065723
TCCAGTC520650.023.68437425
Produced by FastQC (version 0.11.2)