FastQCFastQC Report
Wed 9 May 2018
LZ_M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17617650
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC7337464.164834697022588Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT240650.13659597051820194No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT231560.13143637204735026No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA195550.1109966425715121No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA191030.10843103365091257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA768550.026.9393561
CGTCTGA779850.026.58087716
ATCGGAA778900.026.5571372
ACGTCTG781000.026.54692315
CGGAAGA783800.026.533784
ACACGTC790000.026.26669113
GCACACG791250.026.26442111
CACACGT791900.026.20367212
TCGGAAG791000.026.132393
AGCACAC799050.026.0722110
CACGTCT795700.026.06666214
AGAGCAC802450.026.0037828
GAACTCC801850.025.80125221
GAGCACA808850.025.766339
AACTCCA804600.025.72743222
AAGAGCA812200.025.678337
CAGTCAC787100.025.63511327
GTCTGAA813100.025.55033317
ACTCCAG809950.025.53916723
TCCAGTC810850.025.45785325
Produced by FastQC (version 0.11.2)