FastQCFastQC Report
Wed 9 May 2018
LZ_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17175912
Sequences flagged as poor quality0
Sequence length33
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC12638857.35847389064406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAG338950.19734032172498323Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT198040.11530100992599403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1366650.026.996291
ATCGGAA1375300.026.7777212
CGGAAGA1377750.026.7237154
CGTCTGA1373600.026.59720616
ACGTCTG1376750.026.58437715
TCGGAAG1388250.026.502353
GCACACG1387250.026.45221911
ACACGTC1386300.026.43141413
CACACGT1389300.026.40250412
AGCACAC1393050.026.38470310
AGAGCAC1398050.026.3290218
CACGTCT1392450.026.29819914
AAGAGCA1408950.026.1780077
GAGCACA1406100.026.170619
GAACTCC1388750.026.13593721
GTCTGAA1400350.026.10937317
AACTCCA1392100.026.02645722
CAGTCAC1351000.026.01044827
GAAGAGC1421100.025.961796
ACTCCAG1395850.025.94396823
Produced by FastQC (version 0.11.2)