FastQCFastQC Report
Wed 9 May 2018
LZ_M5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M5.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15823288
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3274892.0696646613523053Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT273790.1730297773762318No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT198990.1257576807045413No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA188290.11899549575284227No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTT176790.11172772687952086No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA169260.10696891821725042No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTT164330.10385325730025265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA351550.026.8868181
CGTCTGA356700.026.36162616
ACGTCTG358900.026.24514815
ATCGGAA359550.026.2359372
CGGAAGA362450.026.077084
ACACGTC364850.025.83932913
CACACGT366750.025.74225612
GCACACG367200.025.72173111
CACGTCT371300.025.38319414
TCGGAAG372850.025.26643
AGCACAC375300.025.26006310
AGAGCAC380400.024.9214028
GAACTCC381200.024.67637321
GAGCACA385450.024.6158969
CAGTCAC373900.024.60341527
GTCTGAA385400.024.47904817
AACTCCA384600.024.47692922
AAGAGCA388550.024.4542267
ACTCCAG385700.024.36513723
TCCAGTC386650.024.24624625
Produced by FastQC (version 0.11.2)