FastQCFastQC Report
Wed 9 May 2018
LZ_M6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M6.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15940575
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC5242173.288570205278041Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT205860.12914214198672258No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAG162570.10198502877091949Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA563450.026.8846861
ATCGGAA571550.026.421662
CGGAAGA571650.026.3967914
CGTCTGA570150.026.32529816
ACGTCTG571150.026.31227915
GCACACG578200.026.00778811
ACACGTC578500.025.99196813
CACACGT580050.025.92251212
TCGGAAG582100.025.8974483
AGCACAC585150.025.76344910
CACGTCT584150.025.724414
AGAGCAC588250.025.65758
GAACTCC589250.025.43071421
GAGCACA595750.025.3367569
AACTCCA593550.025.24304222
GTCTGAA596150.025.2337517
CAGTCAC574950.025.16214627
AAGAGCA602300.025.1418677
ACTCCAG596550.025.09121123
TCCAGTC595850.025.04876925
Produced by FastQC (version 0.11.2)