FastQCFastQC Report
Wed 9 May 2018
LZ_M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18224705
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2674461.4674915176953482Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT290390.15933865596178373No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT267800.14694339359676878No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA239830.13159609442237885No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA222180.12191143834701303No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTT202690.11121716373461189No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT199980.10973017121539141No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTT190330.10443516095322257No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG189100.10376025290944352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA282150.026.7386591
ACGTCTG290000.026.00648515
CGTCTGA290550.025.961916
ATCGGAA292600.025.7456742
CGGAAGA294900.025.686334
ACACGTC297750.025.33410613
CACACGT298000.025.19964812
GCACACG299100.025.15659711
TCGGAAG302600.024.8407363
CACGTCT304100.024.80953614
AGCACAC307600.024.57988510
AGAGCAC309200.024.485268
GAACTCC312750.024.16696421
CAGTCAC306550.023.84825527
GAGCACA318550.023.7519059
AACTCCA319850.023.69003922
ACTCCAG320900.023.52419723
TCCAGTC320950.023.40284525
AAGAGCA327350.023.29267
GTCTGAA326550.023.23200617
Produced by FastQC (version 0.11.2)