FastQCFastQC Report
Wed 9 May 2018
LZ_M8.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M8.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17089474
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4578812.6793159344752215Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT361900.2117677817351195No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA313780.18361009823941918No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA295810.1730948535923341No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT252600.14781028368690577No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG229840.13449214411163268No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG210860.12338589239200691No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG209950.12285340087120294No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT209030.12231505779522531No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATA190950.11173544604122983No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGA185610.10861071557849002No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT181770.1063637183918007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA491600.026.9061641
CGTCTGA501700.026.41129316
CGGAAGA503600.026.3871354
ACGTCTG502500.026.377315
ATCGGAA504100.026.1416782
ACACGTC510100.025.9869513
GCACACG511200.025.89399511
CACACGT510350.025.89217412
TCGGAAG510750.025.7611853
CACGTCT515800.025.68407414
AGCACAC516700.025.67060510
AGAGCAC519700.025.5954728
GAACTCC522150.025.3490721
GAGCACA525550.025.2922469
AACTCCA525450.025.21871222
CAGTCAC509600.025.08283427
ACTCCAG528000.025.06368823
AAGAGCA533150.025.0079967
TCCAGTC529650.024.94745625
GTCTGAA541150.024.57318117
Produced by FastQC (version 0.11.2)