FastQCFastQC Report
Wed 9 May 2018
LZ_M9.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M9.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19492988
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4838172.4820053241709275Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT363820.1866414733338983No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT357610.18345571238231922No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA336510.1726313072167284No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA295360.15152115211890552No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT260150.13345824662694095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258290.13250405735641965No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG257240.13196540212306085No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTT242210.12425493721126797No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTT230460.11822712864749108No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATA224310.11507214799496106No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG213510.10953169416612785No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG208200.10680763770028484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA518550.026.8809241
CGTCTGA529050.026.38061116
ACGTCTG530050.026.36139315
CGGAAGA532100.026.3359324
ATCGGAA532400.026.1421532
ACACGTC536500.026.01931413
GCACACG537700.025.97379511
CACACGT538300.025.92729412
TCGGAAG539550.025.7951813
CACGTCT542500.025.73154314
AGCACAC547150.025.61150710
AGAGCAC553200.025.3460468
GAACTCC552600.025.24684721
GAGCACA559050.025.1073729
AACTCCA556200.025.07712622
CAGTCAC544150.025.01938627
ACTCCAG558000.025.0107423
TCCAGTC560150.024.82846325
AAGAGCA567250.024.7824887
GTCTGAA568350.024.66092317
Produced by FastQC (version 0.11.2)