FastQCFastQC Report
Thu 1 Jun 2017
110414Wax_D11-1461_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename110414Wax_D11-1461_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14562762
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA7000.010.92535612
GATCGGA6400.010.9033331
ATCGGAA6800.010.0053032
AGCGGTT7550.09.00344810
ATGCCGA7550.08.55600625
AATGCCG8550.07.95218724
CGACACG4255.725269E-87.59805153
GCGGTTC9500.07.334255711
GAGCGGT9600.06.90386259
CCGAGAC10850.06.74073828
CGAGACC10900.06.397732729
AGACCGG11250.06.349595531
GACCGGA11150.06.25400632
GAGACCG11450.06.238964630
TCGGAAG12100.06.03977353
CCGGAAC11950.05.835448734
AAGAGCG12800.05.7089837
TGCCGAG12300.05.39154626
GCCGAGA14000.05.223228527