FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6248_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6248_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24061113
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA20400.029.29590233
GACCGGT20400.028.97396932
CGGTAAC20800.028.83776735
CCGGTAA20800.028.62727234
TCTCGTA21400.028.54071444
GATCGGA24300.027.6457631
CGGTTCA25900.026.33086612
ATGCCGA26850.025.35656725
ATCGGAA28200.024.848172
AGCGGTT28050.024.31263710
AATCTCG24950.024.12884542
GAGCGGT29500.023.2707049
GCGGTTC29600.022.96321911
AGACCGG27350.022.2516331
ATCTCGT27100.022.21456543
CGAGACC28250.021.85269729
GAGACCG29500.020.85252830
CCGAGAC29850.020.8280428
TGCCGAG32900.020.82681526
AATGCCG33500.020.71518524
Produced by FastQC (version 0.11.2)