FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6251_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6251_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences26147835
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25600.025.2107181
TCTCGTA21950.024.33528744
ATGCCGA27150.023.06095925
GACCGAA24000.022.98637432
CGGTTCA28250.022.78021812
ATCGGAA29150.022.1592982
AGCGGTT29250.022.15580410
ATCTCGT26600.020.65728643
GAGCGGT33050.019.6083899
GCGGTTC33950.018.88904811
AATGCCG33850.018.88449324
ACCGAAG29350.018.34882233
AGACCGA30600.018.24315531
AAGAGCG36550.018.0302857
CGAGACC31600.017.66583829
CCGAGAC32250.017.44554528
TGCCGAG37200.017.18387426
TCGGAAG37950.017.0750143
CATCTCG32950.016.8091742
GAGACCG34150.016.6031430
Produced by FastQC (version 0.11.2)