FastQCFastQC Report
Thu 1 Jun 2017
G115-M1_GATCAG_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115-M1_GATCAG_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48165028
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18550.011.26740842
TCGTATG19750.010.13705643
CGATCAG23150.09.123133
CGTATGC23300.09.06488644
TCTCGTA20100.08.64712541
ACGATCA25800.08.35643332
GTCACGA27900.08.27945829
TCACGAT30300.07.188021730
CACGATC29000.07.13093831
GATCGGA30650.06.7579221
CGTCTGA34650.06.412145616
GATCTCG31550.06.13650239
ACACGTC36950.06.13217913
ATCGGAA37050.05.937412
ACGTCTG42150.05.58433515
AGTCACG46400.05.310344728
ATCTCGT35000.05.2802340