FastQCFastQC Report
Thu 1 Jun 2017
G144-M8-Index-20_GTGGCC_BC9WPLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M8-Index-20_GTGGCC_BC9WPLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25869874
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12750.013.44597644
TCTCGTA14850.010.80455443
TATCTCG16250.09.8741441
ATCTCGT19600.08.52265242
ACGACGC11250.06.658901716
ATCGACG3800.00498355936.362662341
AGTCACG29700.06.291599828
CGTCGAT4200.0020346186.280855724
CACGACG13650.05.972431715
ACGTGGC33200.05.826739332
CGGTTTT30000.05.72672751
TACACGA14051.8189894E-125.645849713
GTCACGT35750.05.53410629
GCGTCGA6502.8880814E-45.41090623
TCCGATC12806.7848305E-105.32440826
CGACGCT13952.1464075E-105.21211817
CGGATTA8053.8052553E-55.19865371
CGGTTTA12152.932211E-85.0759221
CGGTATT17850.05.05916451
CGTGGCC38750.05.048831533
Produced by FastQC (version 0.11.2)