FastQCFastQC Report
Thu 1 Jun 2017
G4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876000
Sequences flagged as poor quality0
Sequence length35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA3500.014.08274711
CTCGTAT3700.012.53945312
TCGTATG4150.011.52905713
CGTATGC4650.09.9776314
TATGCCG5150.09.29009116
ATGCCGT5350.09.21379117
AGCTCGT6200.08.88527510
GCCGTCT6650.08.06619719
CCGTCTT6400.07.702327320
TGCCGTC6800.07.67558918
CGTCTTC7100.07.556066521
GATCGGA41900.06.99544529
ATCGGAA33400.06.082183429
TCGGAAG28750.06.056494729
AGATCGG49950.06.01275728
CGGAAGA25100.05.954447329
TCTCGAT3507.237858E-45.800608623
GTTGCCG3752.7888868E-45.798552
CGCGTCG3050.0059232535.707156726
GAGCTCG11150.05.3307769
Produced by FastQC (version 0.11.2)