FastQCFastQC Report
Thu 1 Jun 2017
G8.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG8.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005834
Sequences flagged as poor quality0
Sequence length35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11100.020.37500812
CGTATGC11300.020.1477214
TCGTATG11500.019.79227613
GCTCGTA12250.018.4621611
TATGCCG12650.018.45665716
ATGCCGT12850.018.28252217
TGCCGTC14400.016.3144518
AGCTCGT14400.015.90669310
GCCGTCT16000.014.5016619
CCGTCTT16050.014.36684720
GTATGCC17350.013.70721115
CGTCTTC16800.013.54997921
GAGCTCG20900.011.5839769
GTCTTCT31500.07.917535322
TTCTGCT41600.06.13479625
CGGAAGA42950.06.11004074
AGATCGG69450.06.07571229
GCTTGAA18800.06.01607429
AGAGCTC45400.05.9393388
GCTTAGA17650.05.83297529
Produced by FastQC (version 0.11.2)