FastQCFastQC Report
Fri 6 Feb 2015
150123Wax_D15-330_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename150123Wax_D15-330_NA_sequence.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161206209
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA4256890.2640648909497028No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT3637290.22562964680845513No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA3099430.19226492696692593No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT3041080.18864533933677455No Hit
GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA2974910.18454065872859773No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2909450.180480021089014No Hit
GTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTTCAGTGTGCATTTCT2726760.169147331043558No Hit
GAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG2722510.1688836935555007No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA2633360.16335350954131053No Hit
GGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT2524000.15656965173097023No Hit
GGAAAATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTA2503690.155309774699807No Hit
GTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCC2401560.14897441078091478No Hit
GAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAAA2390050.14826041843090548No Hit
GTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTC2365010.14670712838362202No Hit
GTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA2325880.1442798025229909No Hit
GTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGT2310690.14333753112449904No Hit
GTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT2297000.1424883082512039No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA2206920.13690043415139178No Hit
GTCAAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATAT2098810.1301941167787154No Hit
GCCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA2012210.1248221152573596No Hit
CTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGT1965750.12194009227026734No Hit
GACGAAATCACTAAAAAACGTGAAAAATGAGAAATGCACACTGAAGGACC1923490.11931860515372582No Hit
GAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGC1845140.11445837052095184No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1818630.1128138929189756No Hit
GTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCAC1768810.10972344123544274No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC1756690.10897160915185344No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC1745830.10829793782942938No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA1717310.10652877520368957No Hit
GAAAAATGACGAAATCACTAAAAAACGTGAAAAATGAGAAATGCACACTG1696890.10526207461401194No Hit
GAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAAAAATGACG1691470.1049258592763012No Hit
CTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTTCAGT1654810.102651753320494No Hit
GTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTCAAGT1629810.10110094456721576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG549050.041.22142816
TATTCGT408750.040.574721
AGCCGAT561250.040.35760518
AAGCCGA570450.039.73776617
CCGATGA572650.039.56855820
TATAAGC580250.039.08866514
CGATGAC582650.038.9275421
ATAAGCC584300.038.81747415
TCATAAC590900.038.34578329
TTCGGTA624400.037.836825
GACCCTT603550.037.5462842
TTATAAG607150.037.40754313
GCCGATG607300.037.35447719
GATGACG609150.037.30407322
GGATTTA604650.037.289599
TCGGTAT634450.037.2379726
AATTCGG636750.037.17923
ATTCGGT636650.037.1432124
GCGGTCT635800.037.07915539
TCGGGGA637300.037.06717717
Produced by FastQC (version 0.11.2)