FastQCFastQC Report
Fri 6 Feb 2015
150123Wax_D15-331_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename150123Wax_D15-331_NA_sequence.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157429094
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2866830.18210293454397952TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1855020.11783209525426096TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA743600.039.9017561
CTCGTAT759100.038.38969442
ATCGGAA791650.037.4166642
CGTCTGA809550.036.99516
ACGTCTG816950.036.6337415
CGTATGC815350.035.8094144
ATCTCGT830650.035.15735640
TATCTCG287500.033.36974339
GCACACG927750.032.46661411
CGGAAGA940750.032.385124
TCGTATG918150.031.8825743
TCTCGTA952000.030.7750541
TCGGAAG995750.030.0051563
ACACGTC1015850.029.65206313
CACACGT1073850.028.0685712
GATCTCG500200.028.05284539
GTCACGC318600.027.82612629
ACGCCAA335050.026.47987732
CACGTCT1141850.026.38581814
AGTCACG422850.026.1590928
Produced by FastQC (version 0.11.2)