FastQCFastQC Report
Tue 26 Mar 2019
SRR3928574_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3928574_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21802627
Sequences flagged as poor quality0
Sequence length68
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1814610.8322896135406068No Hit
GTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC1513740.694292481360159No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT901580.41351897640591656No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG593830.27236626118494806No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCT452890.2077226748868382No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACC448030.20549358570414472No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGT410260.18816998520407655No Hit
GGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC382440.1754100549442964No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCC351550.16124203748474897No Hit
AGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT328590.15071119640766226No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCC300680.13790998671857294No Hit
GGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGT241750.1108811337276008No Hit
ACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCC240910.11049585905404885No Hit
GGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCC234810.10769803106754061No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAT222280.10195101718705733No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCA218950.10042367830261922No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG218860.10038239887331009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA14250.020.4479311
TACTAGT6950.019.62583758
TCGCGAG12600.018.4513051
GGGGCAA99950.018.4221381
TGCGACG3800.017.13238725
GCACGAT10050.016.6557065
TACACGA6850.015.8384932
TTTTCGA9100.015.3287761
ATGCGAC4050.015.30943524
TCGGGAT9450.015.08958649
CGCTATT17100.015.0459071
CGTCACT31950.014.9412261
CGTAAAC2507.601193E-814.88036154
GATTGGA48400.014.4103161
GCGTAGT10850.013.9991841
GTGTAAT27600.013.9267241
AACTACG47950.013.8344381
CTACTAG10300.013.2430157
CGATCGG11100.012.8457748
ATCGTCA6050.012.80914113
Produced by FastQC (version 0.11.2)