FastQCFastQC Report
Tue 26 Mar 2019
SRR3928579_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3928579_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048571
Sequences flagged as poor quality0
Sequence length68
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1891320.820580156574566No Hit
GTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC1143650.49619128231420506No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT1047820.4546138673846635No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG595100.2581938810870314No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCT472990.20521445776399758No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGT469570.20373063475388561No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACC430700.18686624867112153No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCC390030.16922090310935112No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAT351760.15261683685292246No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCC350450.15204847189875675No Hit
GGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC340720.14782695204835042No Hit
AGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT301150.1306588595015283No Hit
GGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGT275930.11971674946789543No Hit
ACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCC274990.11930891507330324No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT262290.11379881208253648No Hit
TCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC253990.11019772115156293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG2400.023.24953324
TCGCGAG13300.021.2093641
GTTTCGA17650.020.7240771
GGGGCAA121950.019.216661
TTTTCGA11600.018.705871
GATTGGA53450.017.456481
TTCGCGT4500.017.22157919
ATTTCGA11100.015.63878251
ACTACGA62100.015.4741931
CGCTATT19350.014.7382111
GGTTGGA47300.014.6799561
GCGACGT5300.014.0368154
AACTACG49700.013.7839531
TCGCGTT6300.013.77735320
GTTCGTA3853.8198777E-1113.6875631
GCGTAGT10900.013.0818521
CTACGAG72150.013.01782
TACGAGC56750.012.999973
CAATTAC30850.012.8615311
GGCGTAT14250.012.6168531
Produced by FastQC (version 0.11.2)