FastQCFastQC Report
Thu 6 Jul 2017
G155-M10-Index2_CGATGT-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M10-Index2_CGATGT-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21685654
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4400.017.50160812
AGCGTCG4950.016.00069410
GCGTCGT5050.015.24808111
GATCGGA7450.010.9348511
GTCGTGT9250.09.27642113
GAGCGTC9350.09.1768489
ATCGGAA9650.08.4419712
GTCGCCG5507.6215656E-108.40046144
TCGTGTA9850.07.594361314
CGTGTAG11100.07.135527615
AGAGCGT13650.06.7697128
TGGTCGC7608.067218E-96.65792342
GTGGTCG7851.0666736E-76.16541741
GATCTCG9708.54925E-106.12126534
AAGAGCG17050.05.6777187
TCGGAAG16900.05.3415043
GGTCGCC11757.428753E-85.05557443
Produced by FastQC (version 0.11.2)