FastQCFastQC Report
Thu 6 Jul 2017
G155-M13-Index5_ACAGTG-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M13-Index5_ACAGTG-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25090339
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5600.019.25137312
AGCGTCG6100.017.67282710
GCGTCGT6100.016.59075211
GATCGGA8300.012.4686951
GTCGTGT11200.09.42896113
ATCGGAA11450.08.653812
GAGCGTC12600.08.3813169
TCGTGTA12100.08.00001114
GTCGCCG9950.07.73875144
TGGTCGC10700.07.40170342
CGTGTAG14700.07.033939415
AGAGCGT14400.07.0284198
GTGGTCG11400.06.561085741
GATCTCG13400.06.23666334
AAGAGCG18500.06.18433577
TCGGAAG17950.06.01069973
ATCTCGG18950.05.106485435
GGTCGCC16953.6379788E-125.06198543
TCTCGGT18850.05.01712436
CGGTGGT20650.05.00681239
Produced by FastQC (version 0.11.2)