FastQCFastQC Report
Thu 6 Jul 2017
G155-M9-Index1_ATCACG-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M9-Index1_ATCACG-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23857341
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5300.020.34104312
GCGTCGT5550.019.42404711
AGCGTCG6650.016.87275910
GATCGGA8900.012.6174441
ATCGGAA11500.09.1904042
GTCGTGT12050.09.12907813
TCGTGTA13050.08.93513414
CGTGTAG15150.07.986954715
GAGCGTC14700.07.63297849
GATCTCG11700.06.95493534
AGAGCGT16800.06.94099048
GTCGCCG12000.06.78353744
TGGTCGC10900.06.66055542
GTGGTCG11400.06.561201641
TCGGAAG19300.06.1606533
AAGAGCG20850.05.5926827
ATCTCGG16750.05.383226435
Produced by FastQC (version 0.11.2)