FastQCFastQC Report
Thu 6 Jul 2017
RXR_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158410200
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG31950.014.87343812
GCGTCGT33900.013.88816111
AGCGTCG35400.013.54837810
GATCGGA53150.08.7815641
GTCGTGT70100.06.872913
TCGTGTA75350.06.83175614
ATCGGAA70250.06.80074552
GAGCGTC79050.06.1785529
CGTGTAG83450.06.115875715
GTCGCCG60200.05.702893744
TGGTCGC62950.05.48854542
AGAGCGT96900.05.1768328
GTGGTCG68100.05.17026241
Produced by FastQC (version 0.11.2)