FastQCFastQC Report
Thu 6 Jul 2017
RXR_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77982995
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24800.09.7631561
TCGTATG27500.08.6396843
CTCGTAT27350.08.44585442
CGTATGC29250.07.972257644
TCTCGTA29000.07.737852641
ATCGGAA34100.07.22593262
CGTCTGA42550.06.04915316
ATCTCGT45000.05.573323740
ACGTCTG52550.05.274743615
ACACGTC50550.05.222265213
Produced by FastQC (version 0.11.2)