FastQCFastQC Report
Thu 6 Jul 2017
RXR_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77982995
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG15350.013.18571412
GCGTCGT17600.011.75017811
AGCGTCG18950.011.14538910
GATCGGA25100.08.3325341
ATCGGAA32550.06.8312582
TCGTGTA33550.06.75376714
GTCGTGT35000.06.47412613
GAGCGTC37100.06.40449959
CGTGTAG39850.05.79640815
GTCGCCG26700.05.110302444
Produced by FastQC (version 0.11.2)