FastQCFastQC Report
Thu 6 Jul 2017
RXR_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80427205
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26550.09.6175091
CTCGTAT30200.08.52295942
CGTATGC29700.08.369987544
TCGTATG30250.08.14515343
TCTCGTA30000.07.699920741
ATCGGAA35400.07.45761062
CGTCTGA46250.05.993291416
ACGTCTG56500.05.217447815
ATCTCGT47250.05.21479740
ACACGTC52250.05.178655613
TCGGAAG58150.05.0696273
Produced by FastQC (version 0.11.2)