FastQCFastQC Report
Thu 6 Jul 2017
RXR_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80427205
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG15350.015.47903812
GCGTCGT16000.014.8502211
AGCGTCG17800.013.71942410
GATCGGA28550.08.25134851
TCGTGTA38950.07.06001314
GTCGTGT35400.07.022479513
ATCGGAA36600.06.6170442
CGTGTAG41450.06.581086615
GAGCGTC40600.06.17750459
GTCGCCG29550.05.66008144
TGGTCGC31650.05.28437342
AGAGCGT50350.05.19994838
GATCTCG37700.05.074890634
Produced by FastQC (version 0.11.2)