FastQCFastQC Report
Thu 6 Jul 2017
G155-M12-Index4_TGACCA-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M12-Index4_TGACCA-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22480245
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3809.811629E-89.26323612
AGCGTCG5001.1314114E-98.80015310
GCGTCGT5101.0711392E-67.33344511
GATCGGA7002.3288867E-55.66169071
TCGTGTA8603.7305126E-65.37200714
ATCGGAA8702.5508933E-55.0615372
Produced by FastQC (version 0.11.2)