FastQCFastQC Report
Thu 6 Jul 2017
RXR_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140105408
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45300.09.3733651
TCGTATG50600.08.21698843
CTCGTAT53100.08.20318742
CGTATGC54600.07.97755744
TCTCGTA49800.07.81923841
ATCGGAA61600.07.4287812
CGTCTGA79850.05.840855616
ACACGTC87650.05.37114113
ATCTCGT81450.05.104972440
ACGTCTG96950.05.08289615
Produced by FastQC (version 0.11.2)