FastQCFastQC Report
Thu 6 Jul 2017
RXR_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140105408
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG28250.014.0966412
GCGTCGT29200.013.63753411
AGCGTCG32600.012.48520810
GATCGGA49050.08.1700191
TCGTGTA66650.06.70084614
ATCGGAA62650.06.6776442
GTCGTGT63750.06.55722913
CGTGTAG72150.06.37294915
GAGCGTC69350.05.99593649
GTCGCCG51200.05.371280744
AGAGCGT86250.05.0508548
TGGTCGC53850.05.025097442
Produced by FastQC (version 0.11.2)