FastQCFastQC Report
Thu 6 Jul 2017
RXR_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69242490
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG12950.014.27102212
GCGTCGT15550.012.16764911
AGCGTCG16850.011.49006910
GATCGGA22450.08.4343461
ATCGGAA28800.07.03339962
TCGTGTA29300.06.75777114
GTCGTGT31150.06.63907813
GAGCGTC32050.06.58999359
CGTGTAG35650.05.986013415
GTCGCCG23250.05.204635644
Produced by FastQC (version 0.11.2)