FastQCFastQC Report
Thu 6 Jul 2017
RXR_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70862918
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24200.010.0911261
CTCGTAT27250.09.28422942
CGTATGC26750.09.04627844
TCGTATG27000.08.96259243
TCTCGTA26450.08.48391641
ATCGGAA31800.08.0252842
CGTCTGA41500.06.361344316
ACACGTC46200.05.71403713
ACGTCTG50550.05.614082315
ATCTCGT42600.05.57749840
TCGGAAG50950.05.44070243
Produced by FastQC (version 0.11.2)