FastQCFastQC Report
Thu 6 Jul 2017
RXR_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70862918
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG14200.015.95895712
GCGTCGT14700.015.41553611
AGCGTCG16400.014.3542210
GATCGGA25400.09.0156011
GTCGTGT32100.07.470829513
TCGTGTA35150.07.323121514
ATCGGAA32250.07.23722
CGTGTAG37600.06.904418515
GAGCGTC35550.06.80759439
GTCGCCG26000.06.09251244
TGGTCGC27750.05.787434642
AGAGCGT43750.05.73304278
GATCTCG33800.05.40039334
GTGGTCG29700.05.33319541
Produced by FastQC (version 0.11.2)