FastQCFastQC Report
Sun 6 Aug 2017
G155-M5-Index22_CGTACG-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M5-Index22_CGTACG-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20485321
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5600.019.64438412
AGCGTCG6200.017.74292610
GCGTCGT6250.017.60093911
GATCGGA7900.015.0502131
ATCGGAA10150.011.4970612
GTCGTGT10300.010.89359913
GAGCGTC11750.09.3622479
GTCGCCG8550.09.00650444
TCGTGTA12100.08.7272914
AGAGCGT13750.08.6407578
TGGTCGC9000.08.5559942
GATCTCG11450.08.45094534
CGTGTAG13750.08.15995615
TCGGAAG16200.07.74700163
AAGAGCG16900.07.42065867
GTGGTCG10150.07.15284641
GGTCGCC13000.06.93896743
CGGAAGA21000.06.39548874
ATCTCGG15950.06.20455935
TCGGTGG14650.06.006045338
Produced by FastQC (version 0.11.2)