FastQCFastQC Report
Sun 6 Aug 2017
G155-M7-Index25_ACTGAT-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M7-Index25_ACTGAT-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20644484
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3600.017.11265612
AGCGTCG3500.016.34377910
GCGTCGT3700.015.46025611
GATCGGA6100.010.46775151
ATCGGAA7900.08.6401432
GTCGTGT8150.08.3686113
GTCGCCG6553.583409E-107.725569244
TCGTGTA9250.07.373209514
CGTGTAG10150.07.152886415
GAGCGTC9752.1827873E-116.54391059
TGGTCGC7753.4310815E-65.677546542
AGAGCGT11952.0296284E-85.1551528
GTGGTCG8306.4287975E-55.035976441
Produced by FastQC (version 0.11.2)