FastQCFastQC Report
Sun 6 Aug 2017
RXRa_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84003268
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29350.013.3429271
CCGTACG10650.013.01466533
CGTACGT10500.012.15285834
CTCGTAT31950.012.11810544
TCTCGTA33250.010.78433943
ATCGGAA40900.09.7361662
CGTCTGA51300.07.89072916
ATCTCGT51950.07.36848742
ACACGTC57350.07.288295713
ACGTCTG61750.07.05406515
TCGGAAG63050.06.7693353
ACCGTAC21150.06.449555432
TATCTCG41000.06.439004441
CGGAAGA75200.05.61680654
GTACGTA25300.05.565451635
CGTAATC21800.05.44957338
Produced by FastQC (version 0.11.2)