FastQCFastQC Report
Sun 6 Aug 2017
RXRa_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84003268
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG20100.017.51385512
GCGTCGT20800.016.92395411
AGCGTCG22650.015.73597110
GATCGGA31700.012.155511
ATCGGAA38250.010.0164372
GTCGTGT39300.09.46103313
GTCGCCG29000.08.95195444
TCGTGTA40600.08.8326614
GAGCGTC42450.08.81086359
TGGTCGC30900.08.18767742
CGTGTAG46950.07.965987715
AGAGCGT53350.07.50586568
GATCTCG40900.06.828728734
GTGGTCG35850.06.811459541
AAGAGCG63950.06.2960617
TCGGAAG64200.06.20774753
GGTCGCC47100.05.932204743
CGGAAGA79250.05.47328664
ATCTCGG58100.05.3370935
TCGGTGG60750.05.14157238
Produced by FastQC (version 0.11.2)