FastQCFastQC Report
Sun 6 Aug 2017
RXRa_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85614083
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30850.011.48166751
CTCGTAT32900.010.83209744
TCTCGTA32150.010.33227443
ATCGGAA37800.09.6615762
CGTTTCG9800.08.75563133
CGTCTGA49950.08.0158616
ACACGTC55300.07.12081913
ACGTCCG16450.06.5536332
ATCTCGT54300.06.482381342
ACGTCTG62250.06.46729815
AGTCACG69850.06.01534628
TCGGAAG64500.05.93496853
GTCACGT72300.05.75065329
CGTCCGC22350.05.709517533
CGGAAGA76900.05.06355244
Produced by FastQC (version 0.11.2)