FastQCFastQC Report
Sun 6 Aug 2017
RXRa_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85614083
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG19450.017.08067912
AGCGTCG22600.016.25720610
GCGTCGT20500.016.09806611
GATCGGA31100.011.4689091
ATCGGAA40500.09.1875392
GTCGTGT39250.08.85642213
GAGCGTC43050.08.79011259
TCGTGTA40950.08.38111814
CGTGTAG46300.08.07784315
GTCGCCG29350.07.19625544
TGGTCGC32350.06.80069342
AGAGCGT55200.06.616418
AAGAGCG62200.06.3670357
GTGGTCG38000.06.02091141
GATCTCG44900.05.92665534
TCGGAAG65900.05.8802023
CGGAAGA78900.05.21819644
TCGGTGG60750.05.033005738
Produced by FastQC (version 0.11.2)