FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-CTL-rep-2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-CTL-rep-2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12264761
Sequences flagged as poor quality0
Sequence length20-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39050.060.915221
ATCGGAA41050.058.496692
TCGGAAG51950.046.3107953
CGGAAGA56150.043.003454
CTCGTAT33450.034.391342-43
TCTCGTA33650.034.3363440-41
TCGTATG35350.032.60817742-43
CGTATGC35800.032.3133844-45
ATGCCGT37550.029.26081846-47
ATCTCGT40650.028.87743640-41
TGCCGTC39700.027.73040448-49
CATCTCG45950.025.65791138-39
CGTCTGA45300.025.6174616-17
GCCGTCT42450.025.44155348-49
CCGTCTT42750.025.26229350-51
ACACGTC45700.025.22242412-13
TATGCCG45950.024.7188746-47
ACGTCTG48050.024.1384214-15
CGTCTTC44900.023.5860950-51
CACGTCT54400.021.4883414-15
Produced by FastQC (version 0.11.2)