FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-TCP-rep-3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-TCP-rep-3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11357094
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591360.5206965796003802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA14700.023.79390744
GATCGGA24250.017.05422844
AGATCGG33050.012.51276443
ACGACGC4959.094947E-129.77647834
CGACGCT4907.8216544E-119.42731735
TAGATCG14050.09.08064442
CACGACG5051.36788E-98.71171333
TCCGATC6758.367351E-117.821561344
GAGATCG18000.07.699010443
TTCCGAT7356.91216E-117.482031343
GCGACCA7459.276846E-117.3815693
CGACCAC7459.276846E-117.3815694
CAGATCG16450.07.355001444
TACACGA6603.3942342E-97.33284231
ACACGAC6451.686567E-87.161838532
CTACACG7852.233719E-96.725753330
GACGCTC8552.075467E-96.431893336
GGCGACC9651.1277734E-106.3825712
AAGATCG11901.6370905E-115.915179342
ACCGAGA10202.7903297E-95.8228329
Produced by FastQC (version 0.11.2)