FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-TCP-rep-4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-TCP-rep-4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26544106
Sequences flagged as poor quality0
Sequence length20-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA90450.068.005351
ATCGGAA94000.065.819882
TCGGAAG119200.052.0960463
CGGAAGA128250.048.552734
CTCGTAT78500.034.789442-43
TCTCGTA78500.034.44228440-41
ACGGCTA87750.033.68057632-33
TCGTATG82350.033.1629342-43
CGGCTAC88350.033.0366232-33
CGTATGC85750.031.79524244-45
ATGCCGT84300.030.20776646-47
ATCTCGT94350.029.51131440-41
CGTCTGA105300.028.8084116-17
TCACGGC103350.028.6561430-31
GTCACGG109100.027.31128328-29
CATCTCG103700.027.29863438-39
AGTCACG110350.027.14708328-29
ACACGTC112500.027.08784712-13
TGCCGTC96500.026.65785648-49
CCGTCTT95100.026.5953250-51
Produced by FastQC (version 0.11.2)