FastQCFastQC Report
Thu 23 Aug 2018
CAR-CTL-rep-2_3_4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAR-CTL-rep-2_3_4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46690352
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2582010.5530071822975333TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG666200.14268472424452916TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA474250.036.7000732-33
TCACGGC512400.033.89943330-31
AGTCACG531300.032.99647528-29
AATCTCG408600.032.63655540-41
CGGCTAC468700.029.25467932-33
CATCTCG495350.028.0021638-39
CGGAAGA2501600.027.9803184
GAGCACA2511750.027.8445689
TCGGAAG2548250.027.4177883
ATCGGAA2585150.027.0122342
GTCACGG521450.026.48416128-29
AGAGCAC2653350.026.4391148
GATCGGA2635000.026.0409071
CACGGCT532500.025.91213230-31
CTACATC676100.025.81390436-37
GAAGAGC2733850.025.775216
ACATCTC713500.024.64029538-39
CCGTCTT1011500.024.46255750-51
TGCCGTC1029700.024.02411348-49
AAGAGCA2961400.023.8571977
Produced by FastQC (version 0.11.2)