FastQCFastQC Report
Thu 23 Aug 2018
CAR-TCP-rep-1_2_3_4_6_7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAR-TCP-rep-1_2_3_4_6_7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129942165
Sequences flagged as poor quality0
Sequence length50-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4578800.35237214956361546TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA133800.035.4443971
CGGAAGA5162750.029.9714874
TCGGAAG5220500.029.5399863
ATCGGAA5327900.029.0303082
GAGCACA5453250.028.9952059
TGTAATC1798500.027.76829136-37
AGAGCAC5720300.027.7005948
CGTCTGA3901850.027.46623816-17
GATCGGA5392450.027.351131
GAAGAGC5898050.026.9679376
TAATCTC1878200.026.78102538-39
TGCCGTC2864000.026.57578748-49
CCGTCTT2895900.026.32254450-51
TATGCCG2967750.025.89083946-47
CACGTCT4138850.025.88671314-15
CCAGTCA4092250.025.81041726-27
CTTGTAA1950500.025.75666634-35
TCCGATC123450.025.4081528
ACCTTGT1989950.025.32207332-33
ACTGATA1443250.024.73994434-35
Produced by FastQC (version 0.11.2)