FastQCFastQC Report
Thu 23 Aug 2018
CAR-TCP-rep-1_3_4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAR-TCP-rep-1_3_4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61583344
Sequences flagged as poor quality0
Sequence length50-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4578800.743512726428107TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA1036600.16832473403847636TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT889320.14440917661113045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1120150.053.1632353
ATCGGAA1158950.051.7990952
CGGAAGA1155550.051.773864
GATCGGA1121000.048.8236271
CGTATGC1117800.047.08576646-47
TGCCGTC1107450.046.58252350-51
TATGCCG1155800.045.66239548-49
CCGTCTT1150000.045.55786552-53
TATCGGA82850.041.931231
GAGCACA1512700.041.7437329
AGAGCAC1573800.040.1730428
GAAGAGC1589050.039.999736
TGCTTGA1280650.039.57856460-61
ATGCCGT1098500.039.4389448-49
GCCGTCT1111000.038.9019450-51
CGTCTTC1124950.038.17378652-53
CTCGTAT1065250.037.77861844-45
GTATGCC1207400.036.61318646-47
GTCTTCT1432700.036.1086854-55
AAGAGCA1882750.034.0724267
Produced by FastQC (version 0.11.2)