FastQCFastQC Report
Sun 14 Apr 2019
SRR6575147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154426
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.3302552678953026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4850.31406628417494464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.14699597218085036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.13274966650693537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1840.11915092018183467No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1710.11073264864724852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.002151230170.567591
CGCTAAA200.002159542970.499035
CCGCTAA250.00522776956.3992234
TAGGCAT502.9974884E-546.9993555
GTACTAG954.1836756E-1044.5690041
CGACGAA250.001700215637.59948352-53
CATCGTC757.4025756E-637.599489
CGACGAG401.15602E-535.24951624-25
CACTTAT550.002697055534.2145841
TGGGAGA1850.033.0265736
ATGGGAG3150.032.8249445
GTCGACG300.00415659531.33290150-51
TTCGTCT300.00415659531.33290132-33
ACAACGA404.773267E-429.37459858-59
CTAGGCA804.7623424E-429.3745984
CCTTAGG650.00610922328.9508061
GCCCTAA650.00610922328.9508061
GTATCAA10900.028.4859961
CTTAGGA1005.282974E-528.2087482
ATCGTCA505.311609E-528.19961210-11
Produced by FastQC (version 0.11.2)