FastQCFastQC Report
Sun 14 Apr 2019
SRR6575147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154426
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.3542149638014324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4930.3192467589654592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.17807882092393768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.1437581754367788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1730.11202776734487715No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1640.10619973320554828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG3650.042.4902235
GTATCAA10900.038.090361
TGGGACC1001.4014604E-632.8977366
TAGACCT600.00415133731.3311755
TGGGAGA2450.030.6917616
CAACGCA13650.029.953985
TCAACGC13850.029.860764
ATCAACG13700.029.8446583
AACGCAG13850.029.5214336
GTACATG18350.029.3108021
TACATGG18400.029.1269912
CTACACT1154.167783E-628.6067264
TATCAAC14300.028.2729422
GGGAGAT1503.6567144E-828.1980577
ACATGGG18400.027.8404733
ATGGGAC1704.0217856E-927.6451535
GGGACCT856.791945E-427.6451537
GGAGATC1057.3842195E-526.8552918
TTCGTTG350.00882916626.85529184-85
TATTGTA1057.3842195E-526.8552915
Produced by FastQC (version 0.11.2)